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Nemaline myopathy

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A neonate is born floppy with weak cry, poor feeding, and respiratory distress requiring ventilatory support. Facial weakness is prominent (myopathic face, tented upper lip). Muscle biopsy shows characteristic rod-shaped inclusions ("nemaline bodies") on Gomori trichrome stain. CK is normal or only mildly elevated.

Genetically heterogeneous; multiple genes encoding thin-filament proteins of skeletal muscle. The most common:

GeneInheritanceNotes
NEB (nebulin)ARMost common; typically classic/severe forms
ACTA1AD (de novo) > ARSevere and intermediate forms; many de novo
TPM2, TPM3AD/ARVariable severity
TNNT1ARAmish form
CFL2ARRare, severe

Disease severity correlates loosely with gene and mutation type. About a third of cases lack a molecular diagnosis.

  • Hypotonia and weakness, prominent facial and bulbar (feeding/speech) involvement
  • Respiratory weakness is often severe and can be the dominant clinical issue, sometimes out of proportion to limb weakness (diaphragmatic and intercostal involvement)
  • Normal-to-mildly elevated CK (contrasts with muscular dystrophies, where CK is markedly elevated)
  • Slow progression in most subtypes; some forms are static or even mildly improving
  • Cognition normal

Severity ranges from severe congenital (neonatal lethal without ventilation) to childhood/adult-onset mild forms.

  • Muscle biopsy: nemaline rods on Gomori trichrome (red rods on blue-green background); rods are sub-sarcolemmal aggregates of α-actinin and thin-filament proteins
  • EMG: myopathic pattern
  • Genetic testing: multigene panel for congenital myopathies; NEB sequencing is technically demanding (huge gene)
  • Pulmonary function testing (sitting + supine) crucial for management
  • Congenital muscular dystrophies (CMD): high CK, muscle fibrosis on biopsy; biopsy distinguishes
  • Spinal muscular atrophy (SMA): denervation pattern on EMG, SMN1 deletion
  • Myotubular myopathy (X-linked, MTM1): central nuclei, severe respiratory failure in male infants
  • Central core disease: RYR1, central cores on biopsy, malignant hyperthermia susceptibility
  • Congenital myasthenic syndromes: fatigability, decremental EMG response, CHRNE/etc.
  • Respiratory: non-invasive ventilation (BiPAP) often needed nocturnally; tracheostomy in severe cases. Pulmonary follow-up is the lifeline.
  • Feeding: G-tube common in severe forms
  • PT/OT: range of motion, contracture prevention
  • Cardiac: generally not affected (unlike muscular dystrophies)
  • Anesthesia: caution; succinylcholine and depolarizing agents avoided; some forms have malignant hyperthermia susceptibility (RYR1-overlap)

"Nemaline = thread-like rods on biopsy" (from Greek nēma = thread). Combined with normal CK and prominent facial/respiratory weakness, the biopsy + clinical picture is unmistakable.

"Floppy baby + nemaline rods + normal CK": the three-feature trio that points to the diagnosis in most clinical scenarios.