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SRY translocation (46,XX testicular DSD)

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A phenotypically male adult presents with infertility and small testes. Karyotype reveals 46,XX. FISH shows the SRY gene translocated to one X chromosome. The patient has male external genitalia, no uterus, and azoospermia.

Translocation of SRY (sex-determining region Y) from Yp to Xp

  • Most commonly occurs via aberrant X-Y recombination during paternal meiosis
  • SRY is sufficient to initiate male sex determination (testis formation)
  • Results in a 46,XX individual with male phenotype
  • ~10% of 46,XX testicular DSD cases are SRY-negative (other genes involved)
  • Male external genitalia (usually normal)
  • Small testes
  • Azoospermia and infertility (no spermatogenesis; requires AZF region genes on Yq)
  • Gynecomastia (variable)
  • Short stature (no Y chromosome growth genes)
  • Normal to mildly reduced testosterone
  • No uterus or fallopian tubes (AMH present from Sertoli cells)
  • Karyotype: 46,XX
  • FISH or CMA: SRY signal on X chromosome
  • Hormone panel: elevated FSH/LH, low-normal testosterone
  • Testosterone replacement for hypogonadism (supports virilization, libido, and bone health)
  • Counsel that azoospermia causes infertility; the absent Yq AZF region precludes spermatogenesis, so sperm retrieval is not successful and donor sperm or adoption are the options for building a family
  • Address gynecomastia if present (surgical correction); psychological support
  • Genetic counseling: usually de novo from aberrant paternal X-Y recombination; low recurrence risk

"SRY = Sex Region on Y": SRY is the master switch for male development. When it translocates to X, the result is a 46,XX male.

46,XX male vs Klinefelter (47,XXY): Both present with small testes and infertility, but SRY translocation has a normal 46,XX karyotype with SRY on FISH. Klinefelter has 47,XXY and is typically taller.

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